January 4, 2017 at 12:36 pm #1611
A customer approached us with concerns regarding varying contrast results in a mosaic image. The images displayed darkening towards the corners/edges, especially in the upper right hand corners of the images. James (Mike) Scott and Elizabeth Jenkins of the UES team collaborated to come up with two solutions to this challenge – the Koehler Illumination adjustment for the microscope in processing and a software adjustment in post processing, Both adjustments are described here. This technical note spans both Processing and Post-Processing.
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